diff --git a/erpnext/stock/doctype/pick_list/pick_list.py b/erpnext/stock/doctype/pick_list/pick_list.py
index e572540a04c..7b75bb0ffd3 100644
--- a/erpnext/stock/doctype/pick_list/pick_list.py
+++ b/erpnext/stock/doctype/pick_list/pick_list.py
@@ -181,7 +181,11 @@ class PickList(Document):
 			self.item_location_map.setdefault(
 				item_code,
 				get_available_item_locations(
-					item_code, from_warehouses, self.item_count_map.get(item_code), self.company
+					item_code,
+					from_warehouses,
+					self.item_count_map.get(item_code),
+					self.company,
+					picked_item_details=picked_items_details.get(item_code),
 				),
 			)
 
@@ -473,31 +477,38 @@ def get_items_with_location_and_quantity(item_doc, item_location_map, docstatus)
 
 
 def get_available_item_locations(
-	item_code, from_warehouses, required_qty, company, ignore_validation=False
+	item_code,
+	from_warehouses,
+	required_qty,
+	company,
+	ignore_validation=False,
+	picked_item_details=None,
 ):
 	locations = []
+	total_picked_qty = (
+		sum([v.get("picked_qty") for k, v in picked_item_details.items()]) if picked_item_details else 0
+	)
 	has_serial_no = frappe.get_cached_value("Item", item_code, "has_serial_no")
 	has_batch_no = frappe.get_cached_value("Item", item_code, "has_batch_no")
 
 	if has_batch_no and has_serial_no:
 		locations = get_available_item_locations_for_serial_and_batched_item(
-			item_code, from_warehouses, required_qty, company
+			item_code, from_warehouses, required_qty, company, total_picked_qty
 		)
 	elif has_serial_no:
 		locations = get_available_item_locations_for_serialized_item(
-			item_code, from_warehouses, required_qty, company
+			item_code, from_warehouses, required_qty, company, total_picked_qty
 		)
 	elif has_batch_no:
 		locations = get_available_item_locations_for_batched_item(
-			item_code, from_warehouses, required_qty, company
+			item_code, from_warehouses, required_qty, company, total_picked_qty
 		)
 	else:
 		locations = get_available_item_locations_for_other_item(
-			item_code, from_warehouses, required_qty, company
+			item_code, from_warehouses, required_qty, company, total_picked_qty
 		)
 
 	total_qty_available = sum(location.get("qty") for location in locations)
-
 	remaining_qty = required_qty - total_qty_available
 
 	if remaining_qty > 0 and not ignore_validation:
@@ -508,11 +519,44 @@ def get_available_item_locations(
 			title=_("Insufficient Stock"),
 		)
 
+	if picked_item_details:
+		for location in list(locations):
+			key = (
+				(location["warehouse"], location["batch_no"])
+				if location.get("batch_no")
+				else location["warehouse"]
+			)
+
+			if key in picked_item_details:
+				picked_detail = picked_item_details[key]
+
+				if picked_detail.get("serial_no") and location.get("serial_no"):
+					location["serial_no"] = list(
+						set(location["serial_no"]).difference(set(picked_detail["serial_no"]))
+					)
+					location["qty"] = len(location["serial_no"])
+				else:
+					location["qty"] -= picked_detail.get("picked_qty")
+
+			if location["qty"] < 1:
+				locations.remove(location)
+
+		total_qty_available = sum(location.get("qty") for location in locations)
+		remaining_qty = required_qty - total_qty_available
+
+		if remaining_qty > 0 and not ignore_validation:
+			frappe.msgprint(
+				_("{0} units of Item {1} is picked in another Pick List.").format(
+					remaining_qty, frappe.get_desk_link("Item", item_code)
+				),
+				title=_("Already Picked"),
+			)
+
 	return locations
 
 
 def get_available_item_locations_for_serialized_item(
-	item_code, from_warehouses, required_qty, company
+	item_code, from_warehouses, required_qty, company, total_picked_qty=0
 ):
 	sn = frappe.qb.DocType("Serial No")
 	query = (
@@ -520,7 +564,7 @@ def get_available_item_locations_for_serialized_item(
 		.select(sn.name, sn.warehouse)
 		.where((sn.item_code == item_code) & (sn.company == company))
 		.orderby(sn.purchase_date)
-		.limit(cint(required_qty))
+		.limit(cint(required_qty + total_picked_qty))
 	)
 
 	if from_warehouses:
@@ -542,7 +586,7 @@ def get_available_item_locations_for_serialized_item(
 
 
 def get_available_item_locations_for_batched_item(
-	item_code, from_warehouses, required_qty, company
+	item_code, from_warehouses, required_qty, company, total_picked_qty=0
 ):
 	sle = frappe.qb.DocType("Stock Ledger Entry")
 	batch = frappe.qb.DocType("Batch")
@@ -562,6 +606,7 @@ def get_available_item_locations_for_batched_item(
 		.groupby(sle.warehouse, sle.batch_no, sle.item_code)
 		.having(Sum(sle.actual_qty) > 0)
 		.orderby(IfNull(batch.expiry_date, "2200-01-01"), batch.creation, sle.batch_no, sle.warehouse)
+		.limit(cint(required_qty + total_picked_qty))
 	)
 
 	if from_warehouses:
@@ -571,7 +616,7 @@ def get_available_item_locations_for_batched_item(
 
 
 def get_available_item_locations_for_serial_and_batched_item(
-	item_code, from_warehouses, required_qty, company
+	item_code, from_warehouses, required_qty, company, total_picked_qty=0
 ):
 	# Get batch nos by FIFO
 	locations = get_available_item_locations_for_batched_item(
@@ -594,23 +639,26 @@ def get_available_item_locations_for_serial_and_batched_item(
 					(conditions) & (sn.batch_no == location.batch_no) & (sn.warehouse == location.warehouse)
 				)
 				.orderby(sn.purchase_date)
-				.limit(cint(location.qty))
+				.limit(cint(location.qty + total_picked_qty))
 			).run(as_dict=True)
 
 			serial_nos = [sn.name for sn in serial_nos]
 			location.serial_no = serial_nos
+			location.qty = len(serial_nos)
 
 	return locations
 
 
-def get_available_item_locations_for_other_item(item_code, from_warehouses, required_qty, company):
+def get_available_item_locations_for_other_item(
+	item_code, from_warehouses, required_qty, company, total_picked_qty=0
+):
 	bin = frappe.qb.DocType("Bin")
 	query = (
 		frappe.qb.from_(bin)
 		.select(bin.warehouse, bin.actual_qty.as_("qty"))
 		.where((bin.item_code == item_code) & (bin.actual_qty > 0))
 		.orderby(bin.creation)
-		.limit(cint(required_qty))
+		.limit(cint(required_qty + total_picked_qty))
 	)
 
 	if from_warehouses: