diff --git a/erpnext/stock/doctype/stock_reconciliation/stock_reconciliation.py b/erpnext/stock/doctype/stock_reconciliation/stock_reconciliation.py
index d78e3c14048..0201321785f 100644
--- a/erpnext/stock/doctype/stock_reconciliation/stock_reconciliation.py
+++ b/erpnext/stock/doctype/stock_reconciliation/stock_reconciliation.py
@@ -5,7 +5,7 @@
 import frappe
 from frappe import _, bold, msgprint
 from frappe.query_builder.functions import CombineDatetime, Sum
-from frappe.utils import add_to_date, cint, cstr, flt
+from frappe.utils import add_to_date, cint, cstr, flt, get_link_to_form
 
 import erpnext
 from erpnext.accounts.utils import get_company_default
@@ -357,7 +357,6 @@ class StockReconciliation(StockController):
 
 			sl_entries.append(args)
 
-		qty_after_transaction = 0
 		for serial_no in serial_nos:
 			args = self.get_sle_for_items(row, [serial_no])
 
@@ -373,27 +372,16 @@ class StockReconciliation(StockController):
 			if previous_sle and row.warehouse != previous_sle.get("warehouse"):
 				# If serial no exists in different warehouse
 
-				warehouse = previous_sle.get("warehouse", "") or row.warehouse
-
-				if not qty_after_transaction:
-					qty_after_transaction = get_stock_balance(
-						row.item_code, warehouse, self.posting_date, self.posting_time
+				frappe.throw(
+					_(
+						"The Serial No {0} already exists in the warehouse {1}. It cannot be transferred to the warehouse {2}"
+					).format(
+						get_link_to_form("Serial No", serial_no),
+						bold(previous_sle.get("warehouse")),
+						row.warehouse,
 					)
-
-				qty_after_transaction -= 1
-
-				new_args = args.copy()
-				new_args.update(
-					{
-						"actual_qty": -1,
-						"qty_after_transaction": qty_after_transaction,
-						"warehouse": warehouse,
-						"valuation_rate": previous_sle.get("valuation_rate"),
-					}
 				)
 
-				sl_entries.append(new_args)
-
 		if row.qty:
 			args = self.get_sle_for_items(row)
 
diff --git a/erpnext/stock/doctype/stock_reconciliation/test_stock_reconciliation.py b/erpnext/stock/doctype/stock_reconciliation/test_stock_reconciliation.py
index 8f3c9891334..2f4755cf511 100644
--- a/erpnext/stock/doctype/stock_reconciliation/test_stock_reconciliation.py
+++ b/erpnext/stock/doctype/stock_reconciliation/test_stock_reconciliation.py
@@ -1119,6 +1119,33 @@ class TestStockReconciliation(FrappeTestCase, StockTestMixin):
 		active_serial_no = frappe.get_all("Serial No", filters={"status": "Active", "item_code": item_code})
 		self.assertEqual(len(active_serial_no), 5)
 
+	def test_stock_reco_for_serialized_item_with_different_warehouse(self):
+		from erpnext.stock.doctype.stock_entry.test_stock_entry import make_stock_entry
+
+		# Add new serial nos
+		serial_item_code = "Stock-Reco-Serial-Item-11"
+		warehouse = "_Test Warehouse - _TC"
+		serial_warehouse = "_Test Warehouse for Stock Reco1 - _TC"
+
+		self.make_item(
+			serial_item_code, {"is_stock_item": 1, "has_serial_no": 1, "serial_no_series": "SNT-SRS11.####"}
+		)
+
+		make_stock_entry(item_code=serial_item_code, target=warehouse, qty=10, basic_rate=100)
+		stock_entry = make_stock_entry(
+			item_code=serial_item_code, target=serial_warehouse, qty=10, basic_rate=200
+		)
+
+		sr = create_stock_reconciliation(
+			item_code=serial_item_code, warehouse=warehouse, qty=11, rate=200, do_not_submit=True
+		)
+
+		serial_nos = get_serial_nos(stock_entry.items[0].serial_no)
+
+		sr.items[0].serial_no += f"\n{serial_nos[0]}"
+		sr.save()
+		self.assertRaises(frappe.ValidationError, sr.submit)
+
 
 def create_batch_item_with_batch(item_name, batch_id):
 	batch_item_doc = create_item(item_name, is_stock_item=1)