diff --git a/.changes/fix-glob-resource-wix.md b/.changes/fix-glob-resource-wix.md
new file mode 100644
index 000000000..9e534fcc7
--- /dev/null
+++ b/.changes/fix-glob-resource-wix.md
@@ -0,0 +1,5 @@
+---
+"tauri-bundler": patch
+---
+
+Fixes processing of resources with glob patterns when there are nested directories on Windows.
diff --git a/tooling/bundler/src/bundle/windows/msi/wix.rs b/tooling/bundler/src/bundle/windows/msi/wix.rs
index 5ed46a073..4e17feff7 100644
--- a/tooling/bundler/src/bundle/windows/msi/wix.rs
+++ b/tooling/bundler/src/bundle/windows/msi/wix.rs
@@ -799,6 +799,13 @@ fn generate_resource_data(settings: &Settings) -> crate::Result<ResourceMap> {
       .into_string()
       .expect("failed to read resource path");
 
+    // In some glob resource paths like `assets/**/*` a file might appear twice
+    // because the `tauri_utils::resources::ResourcePaths` iterator also reads a directory
+    // when it finds one. So we must check it before processing the file.
+    if added_resources.contains(&resource_path) {
+      continue;
+    }
+
     added_resources.push(resource_path.clone());
 
     let resource_entry = ResourceFile {