diff --git a/.changes/setup-fn-once.md b/.changes/setup-fn-once.md
new file mode 100644
index 000000000..7f06a05ed
--- /dev/null
+++ b/.changes/setup-fn-once.md
@@ -0,0 +1,5 @@
+---
+"tauri": "patch"
+---
+
+`Builder#setup` closure type changed from `Fn` to `FnOnce`.
diff --git a/core/tauri/src/app.rs b/core/tauri/src/app.rs
index 2e0913b83..1804cd47e 100644
--- a/core/tauri/src/app.rs
+++ b/core/tauri/src/app.rs
@@ -677,7 +677,7 @@ impl<R: Runtime> Builder<R> {
   /// Defines the setup hook.
   pub fn setup<F>(mut self, setup: F) -> Self
   where
-    F: Fn(&mut App<R>) -> Result<(), Box<dyn std::error::Error + Send>> + Send + 'static,
+    F: FnOnce(&mut App<R>) -> Result<(), Box<dyn std::error::Error + Send>> + Send + 'static,
   {
     self.setup = Box::new(setup);
     self
diff --git a/core/tauri/src/hooks.rs b/core/tauri/src/hooks.rs
index dfe917a7d..3d9d199a2 100644
--- a/core/tauri/src/hooks.rs
+++ b/core/tauri/src/hooks.rs
@@ -16,7 +16,7 @@ use tauri_macros::default_runtime;
 
 /// A closure that is run when the Tauri application is setting up.
 pub type SetupHook<R> =
-  Box<dyn Fn(&mut App<R>) -> Result<(), Box<dyn std::error::Error + Send>> + Send>;
+  Box<dyn FnOnce(&mut App<R>) -> Result<(), Box<dyn std::error::Error + Send>> + Send>;
 
 /// A closure that is run everytime Tauri receives a message it doesn't explicitly handle.
 pub type InvokeHandler<R> = dyn Fn(Invoke<R>) + Send + Sync + 'static;